Despite significant advancements in vaccines and technologies reducing morbidities and mortalities from infectious diseases like HIV/AIDS and tuberculosis, non-communicable diseases (NCDs) are increasingly contributing to global deaths and disabilities. Lifestyle behaviors (LB) are critically linked to health, wellness, and quality of life. This study assesses the health and wellness lifestyle practices among staff at a tertiary educational institution in the context of prevalent lifestyle-related diseases. An analytical cross-sectional design was employed, using self-administered structured questionnaires on health and wellness completed by 472 respondents. Descriptive and inferential statistics, including Chi-square tests, were utilized for data analysis at an alpha level of 0.05. The respondents, aged between 23 and 64 years, comprised 45.8% males and 54.2% females. General knowledge about participation in moderate physical activity (GKMPA) as a lifestyle habit was good among 60% of respondents. Additionally, there were high levels of stress avoidance strategies (77.7%), avoidance of unsafe sexual practices (76.3%), and adherence to speed limits and seat belt use while driving (58.8%). However, knowledge about the use of fire safety devices was notably poor (28.8%). Significant associations were found between age (X²=12.090, p=0.034) and staff status (X²=11.639, p=0.001) with GKMPA. Gender, age, and religion were significantly associated (p<0.05) with fatty diet restriction, avoidance of destructive habits, and possession of fire extinguishers. Safe sexual orientation was significantly associated with age (X²=20.590, p=0.014) and educational status (X²=10.936, p=0.027). The study concludes that most workers in tertiary institutions exhibit positive lifestyle behaviors towards physical activity as part of wellness. However, there is a need for improved education on fire safety and other critical health practices to ensure comprehensive wellness